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2016 Fiscal Year Final Research Report

Development of applicable methods for advanced gene targeting on mouse Y chromosome

Research Project

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Project/Area Number 15K14369
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionThe University of Tokushima

Principal Investigator

MATSUMOTO Takahiro  徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (70447374)

Project Period (FY) 2015-04-01 – 2017-03-31
KeywordsY染色体
Outline of Final Research Achievements

The Y chromosome is male-specific chromosome and considered to be critical for sexual differentiation and male physiology and behavior. However, its physiological impact has been elusive, owing to the lack of a mouse genetic model. The targeted disruption of the Y-linked genes in mouse ES cells has not been successful, because conventional homologous recombination is hampered by the unique structural features of the Y chromosome. We have overcome this problem and generated floxed mouse line, by using a site-directed insertion vector and CRISPR/Cas9 system. Moreover, we developed multi-copy Y gene knockdown transgenic mice with co-cistronic expression of three miRNA hairpins.

Free Research Field

分子生物学

URL: 

Published: 2018-03-22  

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