Development of novel method for generation of genome editing mice without ex vivo handling of embryos

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14371
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Tokai University
• Principal Investigator: OHTSUKA Masato 東海大学, 医学部, 准教授 (90372945)
• Co-Investigator(Kenkyū-buntansha): 和田 健太 東京農業大学, 生物産業学部, 准教授 (20508113)
• Co-Investigator(Renkei-kenkyūsha): SATO Masahiro 鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 教授 (30287099)
• Research Collaborator: MIURA Hiromi 東海大学, 医学部, 特定研究員 (90599523)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ゲノム編集 / マウス / CRISPR / エレクトロポレーション / in vivo / GONAD

Outline of Final Research Achievements

Animal transgenesis involves three major technical steps; isolation of fertilized eggs, microinjection and subsequent transfer of eggs into recipient females, which require sophisticated equipment as well as highly skilled personnel to perform the task. Genome-editing via Oviductal Nucleic Acids Delivery (GONAD) method developed in this study is a novel technique enabling genome editing in mice through intraoviductal instillation of genome editing components followed by an in vivo electroporation. Since the GONAD does not require ex vivo manipulation of zygotes, it is possible to produce genome-edited mice even by the researchers who do not possess special skills for ex vivo handling and micromanipulation of embryos.

Free Research Field

遺伝子工学、発生工学