2016 Fiscal Year Final Research Report
Development of antigen specific immune cell therapy based on Crispr/Cas9 genome editing system
| Project/Area Number |
15K14404
|
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor therapeutics
|
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| Research Institution | Chiba University (2016)
The University of Tokyo (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | CRISPR / Cas9 / ゲノム編集 / がん抗原 / 細胞傷害性T細胞 |
|---|
| Outline of Final Research Achievements |
We generated T cell receptors-deficient CD8+ T cell delivering modified spCas9 recombinant protein/gRNA complex targeting on T cell receptors by electroporation. We further insert hgp100 specific T cell receptor genes to T cell receptors-deficient CD8+ T cells and generated hgp100-specific CD8+ T cells. These hgp100-specific CD8+ T cells showed good cytotoxic activity against malignant melanoma. We successfully generated the method to delete TCR genes by CRISPR/Cas9 genome editing system and could make antigen-specific cytotoxic T cells by delivering tumor antigen-specific TCR genes to TCR-deficient cells.
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| Free Research Field |
免疫学
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