Development of the system for isolation of T-cell receptor like antibodies against tumor stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14405
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: University of Toyama
• Principal Investigator: Isobe Masaharu 富山大学, 大学院理工学研究部(工学), 教授 (70211050)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: T-cell receptor / Antibody / Tumor stem cell / MHC class I

Outline of Final Research Achievements

In this study, we developed a rapid system for the isolation of TCR-like antibodies targeting cancer stem cells by applying our original high-throughput acquisition technology of antigen-specific antibody from single plasma cells. In order to develop radical curative therapy for cancer, it is strongly required to obtain TCR-like antibodies that recognize antigens that selectively express in both cancer cells and cancer stem cells. In this study, we selected antigens whose expression is also found in cancer stem cells from known cancer antigens, and have succeeded to obtain antibodies recognizing a complex of MHC class I and a tumor antigen-peptide (pMHC) with high affinity and specificity.

Free Research Field

分子遺伝学