Towards sub-single-cell analysis of diploid methylome

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14420
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Kyushu University
• Principal Investigator: ITO TAKASHI 九州大学, 医学研究院, 教授 (90201326)
• Co-Investigator(Renkei-kenkyūsha): MIURA Fumihito 九州大学, 大学院医学研究院, 講師 (50447348)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: DNAメチル化 / PBAT / 単一細胞

Outline of Final Research Achievements

DNA methylation represents a major mechanism for epigenetics, which determines the potential of individual cells. Genome-wide pattern of DNA methylation is termed as the methylome. The methylome differs from one another, depending not only on the type and status of the cell but also between the paternal and maternal genomes. Hence the ultimate methylome analysis is to decipher the complete methylation patterns of the two genomes in the same single cell. While we developed a unique method that has opened the door to the single-cell methylome analysis, here we further improved the method towards the ultimate methylome analysis.

Free Research Field

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