Accurate sequencing of tandem repeat DNA

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14435
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Kyoto University
• Principal Investigator: Koga Akihiko 京都大学, 霊長類研究所, 教授 (80192574)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 塩基配列決定 / 反復配列 / 変異導入 / コンセンサス配列 / コンティグ配列

Outline of Final Research Achievements

The shotgun sequencing method is not powerful enough to obtain accurate sequence data of tandem repeat DNA. Five years ago, I developed an alternative, efficient method. The purpose of the present research was to develop a higher-performance method based on a different principle.
The new method I established was demonstrated to produce sequence data of which the accuracy is as high as those obtained by the previous method, and far higher than those by the shotgun method. However, its performance has not yet exceeded that of the previous method in that it requires a longer period and higher cost for each sequencing assay.
At present, the new method needs additional improvements. During the two-year period, however, basic experimental data to be used toward this goal accumulated.

Free Research Field

Molecular genetics