Functional analyses of condensins on chromatin templates

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14455
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hirano Tatsuya 国立研究開発法人理化学研究所, 平野染色体ダイナミクス研究室, 主任研究員 (50212171)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 染色体 / クロマチン / ヌクレオソーム / コンデンシン

Outline of Final Research Achievements

Two multiprotein complexes, condensins I and II, are central to mitotic chromosome assembly. The current project was aimed at understanding how condensins interact with chromatin templates and induce their conformational changes. To this end, we established protocols for isolating “mini-chromosomes” assembled in Xenopus egg cell-free extracts, either on beads or in solution, and then analyzes the behavior of condensins on those mini-chromosomes. We found that binding of condensin II to mini-chromosomes competes with nucleosome assembly. Such a property was not observed for condensin I.

Free Research Field

分子生物学