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2016 Fiscal Year Final Research Report

Development of high-throughput system of visible immunoprecipitation assay

Research Project

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Project/Area Number 15K14456
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionKyoto University

Principal Investigator

Nakayama Kazuhisa  京都大学, 薬学研究科, 教授 (40192679)

Co-Investigator(Renkei-kenkyūsha) KATOH Yohei  京都大学, 大学院薬学研究科, 助教 (90568172)
Project Period (FY) 2015-04-01 – 2017-03-31
Keywordsタンパク質間相互作用 / GFP / Nanobody / 繊毛内タンパク質輸送複合体
Outline of Final Research Achievements

The visible immunoprecipitation (VIP) assay is a convenient, flexible method to detect protein-protein interactions. In this study, I attempted to improve the VIP assay to detect protein-protein interactions on a microplate and enable high-throughput screening.
By fusing polystyrene (PS)-affinity peptide sequence to anti-GFP-Nanoboy, I successfully immobilized it to PS microplate. The sensitivity was further enhanced by ELISA, through optimizing the concentrations of the secondary and tertiary antibodies.

Free Research Field

分子細胞生物学

URL: 

Published: 2018-03-22  

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