Studies on small motion of endomembranes affecting reactions in the narrow space

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14462
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Fukushima Medical University
• Principal Investigator: WADA IIKUO 福島県立医科大学, 医学部, 教授 (40182969)
• Research Collaborator: Yoshida Hiroshi ; Arai Seisuke ; Inoue Naokazu ; Hashimoto Hitoshi ; Takeuchi Mayumi
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 小胞体 / 揺らぎ

Outline of Final Research Achievements

As reactions confined in the endomembrane system of eukaryotes occur in a small volume, the volume change may affect the kinetics. When endomembrane　motion was observed at high frame rates, we noted small nonhomogeneous fluctuation of the endoplasmic reticulum (ER). Quantitative analyses using the trapped aggregates in the ER lumen revealed that this motion was unique to the ER and that the rate was nearly consistent in all cells examined. This motion was solely caused by the activity of the ER membranes and demonstrated no temperature dependence. By forcefully repressing by microtubules, we found that cargo motion was facilitated by the structure fluctuation.

Free Research Field

細胞生物学