Construction of photoregulation system of protein kinase by LOV domain

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14479
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: TOKUTOMI SATORU 大阪府立大学, 理学(系)研究科(研究院), 客員教授 (90142009)
• Co-Investigator(Renkei-kenkyūsha): IHARA HIDESHI 大阪府立大学, 大学院・理学系研究科, 准教授 (60254447)
• Research Collaborator: KATSURA HITOMI 大阪府立大学, 大学院・理学系研究科, 研究員
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: フォトトロピン / 青色光受容体 / セリン/スレオニンキナーゼ / 活性光制御

Outline of Final Research Achievements

Phototropin (phot) is a plant blue light receptor that mediates phototropic responses et al. Phot molecule has two photoreceptive domains named LOV1 and 2 in the N-terminal half and the C-terminus forms Ser/Thr kinase and acts as photoregulated protein kinase. It has been shown that conformational changes in Ja-helix residing between LOV2 and kinase domains play a crucial role in the kinase photoregulation. Mimicking this photoregulation mechanism, we tried to create a new photoregulation system of protein kinase A (PKA) using LOV2, Ja-helix and PKA inhibitor peptide (PKI). We succeeded in the light-induced prohibition of PKA by about 60% and also obtained new information regarding the molecular mechanism of the photoregulation. However, this was not applicable to ERK that is a key PKA in the MAP kinase pathway regulating cell growth and mitosis.

Free Research Field

光生物学