Real time three dimensional imaging of chemiluminescence with Fresnel incoherent correlation holography

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14487
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Osaka University
• Principal Investigator: Nagai Takeharu 大阪大学, 産業科学研究所, 教授 (20311350)
• Research Collaborator: Brooker Gary Johns Hopkins大学, Biomedical Engineering, 教授 ; TAHARA ITSUKI 関西大学, システム理工学部, 助教 (50709095)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 化学発光 / ホログラフィー / 超解像顕微鏡 / イメージング / ３次元

Outline of Final Research Achievements

We proceeded experiment toward development of “Superresolution chemiluminescence holographic microscopy” enabling chemiluminescence image acquisition with high speed, three-dimension and superresolution quality, by applying chemiluminescent protein with Fresnel incoherent correlation holography (FINCH) and wavefront correction system. By observing HeLa cells expressing chemiluminrescent protein “Nano-lantern” using FINCH, we could reconstruct the chemiluminescent tomographic images with as good quality as the fluorescence ones. To correct wavefront distortion of light, we constructed a wavefront correction system and evaluated the superior performance. To achieve high speed and multi-color acquisition of the chemiluminescent holograms, we developed color palettes of chemiluminescent protein “eNano-lantern” with approximately 10-fold improvement in the brightness compared with previous Nano-lantern.

Free Research Field

生物物理学