Investigating protein behaviours in mitochondria by in-organelle NMR

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14494
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: ITO Yutaka 首都大学東京, 理工学研究科, 教授 (80261147)
• Co-Investigator(Kenkyū-buntansha): 美川 務 国立研究開発法人理化学研究所, その他, 研究員 (20321820)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: In-cell NMR / In-organelle NMR / ミトコンドリア / 蛋白質

Outline of Final Research Achievements

Protein behaviours in eukaryotic organelles were investigated by extending the in-cell NMR approaches to be available for the analysis of proteins localised in organelles, particularly mitochondria.
A model protein with a mitochondrial targeting signal was incorporated into cultured human HeLa.S3 cells by electroporation, and then the mitochondria faction was isolated for NMR experiments. Well-resolved in-mitochondria NMR spectra showed that the proper 3D structure was formed, while different effects of the mitochondria matrix environment to the conformation and dynamics of proteins was suggested from the observed spectral differences when compared with the samples in cytosol or in diluted solution suggested.

Free Research Field

構造生物学