2016 Fiscal Year Final Research Report
Control of cell differentiation by the cytoplasmic pH change
| Project/Area Number |
15K14498
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Morimoto Yusuke 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50631777)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | シグナル伝達 / 細部内pH / 蛍光イメージング / 細胞分化 / 光遺伝学 |
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| Outline of Final Research Achievements |
Intracellular pH plays important roles in the signal transduction for cell differentiation. However it remains unknown how intracellular pH change works in the cell differentiation. To investigate the role of intracellular pH, we constructed Dictyostelium discoideum strain expressing pH-sensitive fluorescent protein and observed cytoplasmic pH changes in the multicellular stage by fluorescence microscopy. And we could regulate the cytoplasmic pH of Dictyostelium cells expressing channelrhodopsin by photostimulation. We will try to control the cell differentiation by photostimulation.
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| Free Research Field |
生物物理
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