Development of a method to isolate molecules in cellular structures where fluorescent proteins localize

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14500
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: Matsuda Atsushi 国立研究開発法人情報通信研究機構, 未来ICT研究所フロンティア創造総合研究室, 主任研究員 (20585723)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: バイオイメージング / 低温工学

Outline of Final Research Achievements

This study is intended to develop a method to isolate frozen cellular micro-compartments relying on fluorescence of localized proteins. We examined methods for crushing ice into small particles, which is suitable for sorting by fluorescence, using bullet fracturing and beads milling. Also, we have designed and built a sorter microscope to sort ice particles containing cellular micro-compartments using fluorescence under low temperature. Simultaneously we have developed a system that prevents solidification of water vapor on the optical components. Using this microscope, we were able to observe ice particles made by bullet fracturing under low temperature, and found that these ice particles are below 10 micrometers in diameter.

Free Research Field

生物物理