2016 Fiscal Year Final Research Report
Development of live-cell assay system for monitoring phospholipid transport
| Project/Area Number |
15K14507
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | リン脂質 / オルガネラ |
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| Outline of Final Research Achievements |
It has been largely unknown how phospholipid, major constituents of cellular membranes, travel within cells due to lack of reliable assay system to analyze phospholipid transport. To overcome this shortcoming, we tried to develop a novel assay system for directly measuring phospholipid transport in cells. By expressing PssA, which is the E. coli PS (phosphatidylserine) synthetic enzyme in a number of cellular compartments within yeast cells, we succeeded to observe PS transport from various organelles to mitochondria.
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| Free Research Field |
分子細胞生物学
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