Visualization of DNA binding proteins bound to specific loci in vivo

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K14514
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: National Institute of Genetics
• Principal Investigator: Sawa Hitoshi 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (80222024)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: エピジェネティックス / C. elegans / イメージング / Wnt / 非対称細胞分裂 / ヒストン修飾

Outline of Final Research Achievements

Using C. elegans, we tried to visualize chromatin status of a specific gene (egl-18). We established a reporter array containing hundreds copies of the gene promoter and the lacO sequence, and measured changes of nuclear positions of the array during cell differentiation using lacI::GFP, enabling us to visualize dynamics of a single gene locus during development. In addition, by immunostaining using anti-modified histone antibodies, we succeeded to visualize dynamic changes of histone marks on this gene during development.

Free Research Field

発生生物学