Identification of blastocoel fluid components and its function in early development

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14521
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: KONDO Mariko 東京大学, 大学院理学系研究科(理学部), 准教授 (70372414)
• Co-Investigator(Kenkyū-buntansha): 高橋 秀治 広島大学, 両生類研究センター, 特任准教授 (90447318)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アフリカツメガエル / 胞胚腔 / 初期発生 / プロテオーム解析

Outline of Final Research Achievements

The role of the blastocoel in early embryos are unknown. In this study, we hypothesized that it functions as the space which is used for signaling between tissues or for cell proliferation, and tried to verify this using proteomics of the blastocoel fluid.
We identified proteins that are involved in iron ion transport or homeostasis, proteinases, etc. However, we could not detect ligand proteins involved in cell signaling, and our hypothesis was not supported. The results suggested that the blastocoel may be utilized as a pool to digest unnecessary proteins during early embryogenesis.

Free Research Field

発生学