2017 Fiscal Year Final Research Report
Blood flow-mediated cell behaviors during vascular remodeling
| Project/Area Number |
15K14526
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOKUBU Hiroshi 京都大学, 大学院理学研究科, 教授 (50202057)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 血管 / リモデリング / 血流 / トリ杯 / 細胞挙動 / 定量化 |
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| Outline of Final Research Achievements |
When the initial vascular network is formed during embryogenesis, primitive vascular plexus undergoes remodeling. Although it has been known that blood flow is important for the vascular remodeling, how the remodeling proceeds remained poorly known. In this study, toward understanding these mechanisms, we aimed at establishing a novel experimental system in which the blood flow and behaviors of endothelial cells were quantitatively measured in vivo. Using vitelline arteries that undergo massive remodeling in a 2-D structure of early chicken embryos, we succeeded in live imaging of remodeling vasculature. When we artificially stopped/attenuated the blood flow leaving the hear beat unaffected, the remodeling of vitelline artery was compromised. EGFP-labeled endothelial cells displayed dynamic behavior that was dependent on the blood flow. Dynamics of blood flow in micro-environment were also measured, providing a system in which vascular remodeling can be analyzed at the cellular level.
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| Free Research Field |
発生生物学、細胞生物学、分子生物学、血管生物学
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