Development of a technology that can visualize interactions between genomic DNA and proteins in nuclei in situ

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14529
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyushu University
• Principal Investigator: Chikara Meno 九州大学, 医学研究院, 教授 (20311764)
• Co-Investigator(Renkei-kenkyūsha): OKI Shinya 九州大学, 医学研究院, 助教 (90452713)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 転写因子 / ゲノムDNA / PLA法

Outline of Final Research Achievements

Although chromatin immunoprecipitation is a useful assay that probes protein-genomic DNA interactions in nuclei, it requires a lot of cells and the purification of specific cells by FACS when tissues are examined. We attempted to develop a general method that detects binding of specific proteins to genomic DNA on tissue sections. We developed the method by examining binding of Pou5f1 to Pou5f1 enhancer in P19 cells as a model case, and obtained results that suggest their interactions. In order to make the method reliable, however, further investigation is required.

Free Research Field

発生生物学