2017 Fiscal Year Final Research Report
Analysis of mouse embryos in diapause
| Project/Area Number |
15K14533
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
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| Research Collaborator |
KAMEMIZU Chizuru
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 発生休止 / 細胞周期 / 着床 |
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| Outline of Final Research Achievements |
Many mammalian species undergo diapause and suspend embryonic development at the blastocyst stage before implantation, which is also known as delayed implantation. We analyzed mouse embryos during diapause by cell cycle, identified by the expression of a transgenic cell cycle marker Fucci2 and also by Ki67 antibody staining. We found that diapause can be categorized into four stages. Cell differentiation status was maintained during these stages, and entrance into and exit from dormant status varied depending on cell layers and its location in an embryo. These results suggest that the initiation of embryonic diapause has multiple steps and the mechanisms involved in dormancy may be distinct between embryonic regions.
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| Free Research Field |
発生生物学
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