2017 Fiscal Year Final Research Report
Analysis of polyspermy block system based on multiple pollen tube attraction.
| Project/Area Number |
15K14541
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Yokohama City University (2016-2017)
Nagoya University (2015) |
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Principal Investigator |
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| Research Collaborator |
HIGASHIYAMA Tetsuya
KAWASHIMA Tomokazu
Berger Frederic
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 重複受精 / 花粉管 / 卵細胞 / 中央細胞 / 多花粉管拒否 / 多精拒否 |
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| Outline of Final Research Achievements |
Zygote of flowering plants prevents multiple fertilizations caused by additional pair of sperm cells from second pollen tube. To examine the mechanism of polyspermy block, we analyzed patterns of released pollen tube contents (PTC) including sperm cells in an Arabidopsis mutant that frequently attracts second pollen tube. Contrary to our expectation, fluorescent signals from the PTC were frequently found in the endosperm but not in the fertilization zone. As this unique phenomenon seems to avoid polyspermy through a rerouting of sperm destination, we further analyzed other mutants that attract multiple pollen tubes and found possible involvement of synergid-endosperm fusion (SE fusion) during early embryogenesis. To prove the relevance of SE fusion in the unusual PTC reception, we tried to isolate mutant plants defective in the SE fusion and finally obtained one.
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| Free Research Field |
植物生殖
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