Chemical library screening to identify novel key regulators of organelle distribution in plants

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14542
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: SATO YOSHIKATSU 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (30414014)
• Co-Investigator(Renkei-kenkyūsha): SATO Ayato 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (10512428) ; KUWATA Keiko 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 化合物ライブラリースクリーニング

Outline of Final Research Achievements

Proper distribution of organelles is essential for proper cell function in eukaryotic cells. In this study, we performed chemical library screening to identify novel key regulators of organelle distribution in plants. As a result of high-throughput but careful screening design, we obtained 9 candidates from 20,000 chemicals, which can be divided into 4 groups by structure-activity relationships. We focused 1 of these groups and evaluated by time-lapse analysis. Then, we confirmed 5 candidates having common structures. Furthermore, we succeeded to identify the essential and non-essential part from hit compounds and to synthesize the dimer compound having strong effect on organelle distribution. Therefore, we conclude that we could obtain important tools and information to elucidate the basic mechanism of organelle distribution in plants.

Free Research Field

植物細胞生物学