2016 Fiscal Year Final Research Report
Molecular analysis of plant kinetochore
| Project/Area Number |
15K14545
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | シロイヌナズナ / キネトコア / タンパク質複合体 / インタラクトーム / GFP |
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| Outline of Final Research Achievements |
Kinetochores are the fundamental link between chromosomal domains called centromeres and spindle microtubules in all eukaryotes. This link, composed by multiprotein complexes, is essential for precise chromosome segregation into daughter cells and thus keeps genetic stability. In yeast and animal cells, many components of kinetochores have been identified and characterised. On the contrary, in plants, number of identified kinetochore protein is largely limited. To identify comprehensively the kinetochore components in plants, we performed interactome analysis in Arabidopsis plants that stably express centromere proteins fused with a fluorescent protein. We found that newly identified proteins, which include kinase- associated protein and unknown proteins, are likely associated with plant kinetochore structure. Together, these findings contribute to a unified view of how plant kinetochore functions as a regulator of chromatin segregation.
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| Free Research Field |
植物細胞生物学
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