Does the establishment of mycoheterotrophic symbiosis between orchids and mycorrhizal fungi require the genes of common symbiotic pathway necessary for mutualistic plant-mycorrhizal symbioses?

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14550
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tottori University
• Principal Investigator: Kaminaka Hironori 鳥取大学, 農学部, 准教授 (40397849)
• Co-Investigator(Renkei-kenkyūsha): YAMATO Masahide 千葉大学, 教育学部, 准教授 (00571788)
• Research Collaborator: IMAIZUMI Haruko (ANRAKU Haruko) ; MIURA Chihiro ; YAMAMOTO Tatsuki ; FURUI Yuki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ラン / 菌根共生 / トランスクリプトーム / 菌寄生性 / 共生菌

Outline of Final Research Achievements

The mycorrhizal symbiosis in orchids has a feature that carbon is provided by mycorrhizal fungi, whose nutrition mode is termed as mycoheterotrophy and opposite to the general mutual symbioses. It has been reported that the genes of common symbiotic pathway are required for mutualistic plant-mycorrhizal symbioses, but the information about the molecular mechanisms of the relationship between orchids and their mycorrhizal fungi is limited. In this study, we conducted the transcriptome analysis and expression analysis of marker genes for mutual symbiosis using symbiotic germination of Bletilla striata (Orchidaceae) and the complementation experiment using CCaMK, which is a key gene for common symbiotic pathway. Our results partially demonstrated that the establishment of mycoheterotrophic symbiosis between orchids and mycorrhizal fungi require the genes of common symbiotic pathway necessary for mutualistic plant-mycorrhizal symbioses.

Free Research Field

植物微生物相互作用