Light-induced regulation of flagellar and ciliary motility by a novel photoreceptor protein

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14566
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal physiology/Animal behavior
• Research Institution: University of Tsukuba
• Principal Investigator: INABA Kazuo 筑波大学, 生命環境系, 教授 (80221779)
• Co-Investigator(Renkei-kenkyūsha): SHIBA Kogiku 筑波大学, 生命環境系, 助教 (70533561) ; HIROSE Keiko 国立研究開発法人産業総合研究所, バイオメディカル研究部門, 主任研究員 (90357872)
• Research Collaborator: KUTOMI Osamu ; JOKURA Kei
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: DYBLUP / ダイニン / BLUP / 光応答 / 鞭毛 / 繊毛 / 精子 / クラミドモナス

Outline of Final Research Achievements

This research project was carried out to clarify the molecular nature and mechanism of a novel dynein photoreceptor DYBLUP. The 2 years project showed (1) the molecule of f/I1 dynein form ascidian sperm is similar to that from Chlamydomonas flagella, (2) ascidian DYBLUP is bound to the motor region of f/I1, (3) the protein product of a gene (MOT7) orthologous to DYBLUP is also present in Chlamydomonas flagella, (5) a Chlamydomonas mutant lacking DYBLUP/MOT7 shows suppressed positive phototaxis and the turn movement in photoshock reaction.

Free Research Field

分子細胞生物学