Base-excision restriction enzymes: structure, function and epigenetics

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K14572
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: KOBAYASHI Ichizo 東京大学, 新領域創成科学研究科, 教授 (30126057)
• Co-Investigator(Renkei-kenkyūsha): YANO Hirokazu 東京大学, 大学院新領域創成科学研究科, 特任助教 (20646773) ; IDE Hiroshi 広島大学, 理学(系)研究科(研究院), 教授 (30223126) ; FUKUYO Masaki 千葉大学, 大学院医学研究院, 特任助教 (40639085) ; KOJIMA Kenji 東京大学, 大学院新領域創成科学研究科, 特任助教 (90748774)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: 制限修飾系 / 制限酵素 / 塩基除去 / DNA切断酵素 / キャンピロバクター / ピロリ菌 / APリアーゼ / APエンドヌクレアーゼ

Outline of Final Research Achievements

We earlier reported that R.PabI from hyperthermophilic arachaeon belongs to the base-excision class of restriction enzyme (DNA N-glycosylase). We purified its homologs from Campylobacterales order and demonstrated its base excision and AP lyase (DNA strand breakage) activity. They show restriction activity towards phage in Escherichia coli. For R.PabI, AP endonuclease mutants decrease the restriction.
We analyzed their molecular evolution and demonstrated they represent mobile genes. In Helicobacter pylori, the gene was lost in some East Asian strains and all Amerind strains. Recombination between East Asian strains and Indian strains took place in this locus.

Free Research Field

分子遺伝学