An approach toward developing mitochondrial genome-editing system in rice cells containing enlarged mitochondria

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14623
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Tohoku University
• Principal Investigator: TORIYAMA Kinya 東北大学, 農学研究科, 教授 (20183882)
• Co-Investigator(Renkei-kenkyūsha): KAZAMA TOMOHIKO 東北大学, 大学院農学研究科, 助教 (30431464)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物

Outline of Final Research Achievements

To establish genome-editing system in rice mitochondria, we developed a rice cell suspension lines with enlarged mitochondria mediated by dominant-negative dynamin-like transgene. We introduced a GFP gene using particle gun. We did not, however, detect introduced GFP expression in the mitochondria. We constructed a mini-circle DNA containing a GFP expression cassette and plasmid-like B1 DNA, which was stably present in mitochondria of certain cultivars. The resulting vector can be used as a basic tool for genome-editing experiments in rice mitochondria.

Free Research Field

植物分子遺伝学