Synthesis of a specific RNA-binding protein via the artificial evolution of Rf-PPR homologs

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14626
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: The University of Tokyo (2017); Nara Institute of Science and Technology (2015-2016)
• Principal Investigator: Fujii Sota 東京大学, 大学院農学生命科学研究科(農学部), 助教 (90716713)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 稔性回復遺伝子 / 人工進化 / RNA編集

Outline of Final Research Achievements

In the age of genome-editing, technologies that enable engineering of RNA expression is demanded. In this project, I aimed to establish such system by using the Rf-PPR protein, an RNA-binding protein with great diversity and ubiquitously present in angiosperm species. First of all, I established a system that visualizes the binding of a protein to an RNA sequence in E. coli. I have also performed functional characterization of an Rf-PPR homolog. The RFL2 was shown to be involved in the cleavage of the RNA transcript of the mitochondrially-encoded orf291.

Free Research Field

植物分子遺伝学