Studies on the replication mechanism of a mini circle found in transplastomic plants and a vector development

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14629
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Kyoto Sangyo University
• Principal Investigator: TERACHI Toru 京都産業大学, 総合生命科学部, 教授 (90202192)
• Co-Investigator(Kenkyū-buntansha): 木村 成介 京都産業大学, 総合生命科学部, 教授 (40339122) ; 山岸 博 京都産業大学, 総合生命科学部, 教授 (10210345)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 葉緑体 / 形質転換 / タバコ / ミニサークル / 斑入り / apx / プラスミド

Outline of Final Research Achievements

In order to clarify the replication mechanism of a “mini circle” found in the chloroplast of transplastomic tobacco plants, plasmids containing a different chloroplast DNA fragment from the mini circle and the antibiotic resistant gene aadA were constructed. Then, the plasmids were introduced into tobacco leaves by particle bombardment experiments. All of three plasmids constructed with a different chloroplast DNA fragment produced antibiotic resistant shoots, showing that these plasmids could replicate in the chloroplast. Deletion mutants from the plasmid #2A also yielded antibiotic resistant shoots, indicating a part of the #2A fragment contained replication origin that were functional in the chloroplast.

Free Research Field

植物分子遺伝学