Development of DNA markers for identification of intra-cultivars and for discrimination of production region in fruit trees

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14649
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Shizuoka University
• Principal Investigator: Yahata Masaki 静岡大学, 農学部, 准教授 (60420353)
• Co-Investigator(Kenkyū-buntansha): 松山 知樹 国立研究開発法人理化学研究所, 主任研究員研究室等, 専任研究員 (30291090)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: イオンビーム / 果樹 / カンキツ / DNAマーカー / 突然変異

Outline of Final Research Achievements

In order to develop DNA markers for identification of intra-cultivars and for discrimination of production region, ion-beam-irradiation was performed for Citrus seedlings except roots. Seedlings obtained from 0 - 30 Gy treatments showed normal growth similar to thier controls. However, 50 Gy-irradiated seedlings showed dwarf growth, variegation and mutations of leaf shape. DNA polymorphism was detected from one of them. After grafting propagation, all clones stably maintained the same of DNA polymorphism.

Free Research Field

果樹園芸学