Elucidation of nutrient starvation signal transduction during bud dormancy in gentian

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14658
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Takahashi Hideyuki 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (00455247)
• Research Collaborator: FUJITA KOUHEI 公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手 ; YOSHIDA CHIHARU 公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 花卉 / 休眠

Outline of Final Research Achievements

Dormancy caused by nutritional starvation is a specific regulatory system of bacteria and fungi. This dormancy is mediated by stringent response that is regulated by RelA/SpoT Homologs (RSH). However, we previously found that gentian plantlets cultured under P deficient condition produced dormant organ, indicating the possibility that plants also possess a dormancy mechanism regulated by stringent response. In this study, we identified two RSH genes (RSH1 and CRSH) and found that the expression level of these genes increased by P starvation in gentian plantlets. Furthermore, we identified 13 variants of RSH1 using a next generation sequence technique. Interestingly, the expression ratio of functional variant was higher in plantlets cultured under P deficient condition. The ratio was also higher in dormant organ of field grown gentian plants. These results imply that P concentration-dependent regulation of RSH may be involved in dormancy mechanism in gentian.

Free Research Field

植物代謝