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2017 Fiscal Year Final Research Report

Development of a system to confer plant virus disease tolerance through the regulation of reactive oxygen species production in chloroplasts

Research Project

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Project/Area Number 15K14664
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant protection science
Research InstitutionEhime University

Principal Investigator

KOBAYASHI Kappei  愛媛大学, 農学研究科, 教授 (40244587)

Research Collaborator KOSAKA Naomi  
YAMASHITA Mei  
OKAMOTO Maiko  
BHOR Sachin Ashok  
Project Period (FY) 2015-04-01 – 2018-03-31
Keywords植物ウイルス病 / 遺伝子組換え植物 / 活性酸素種 / 葉緑体
Outline of Final Research Achievements

The present study aimed to generate plants tolerant to plant virus diseases. To this end, we designed a genetically modified plant system to express timely the gene(s) regulating the reactive oxygen species (ROS) production in chloroplasts, which is known to have roles in plant disease development. We established a cheap method to introduce foreign genes into chloroplast and introduced genes that can regulate ROS production into tobacco chloroplasts. Through the examination of a switching gene that turns on the foreign gene in chloroplasts, we found that both protein and RNA structures are important to enable the switching gene product for its efficient transport into chloroplasts.

Free Research Field

植物ウイルス病の発症機構の解析と耐病性分子育種

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Published: 2019-03-29  

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