2017 Fiscal Year Final Research Report
Development of a system to confer plant virus disease tolerance through the regulation of reactive oxygen species production in chloroplasts
Project/Area Number |
15K14664
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant protection science
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Research Institution | Ehime University |
Principal Investigator |
|
Research Collaborator |
KOSAKA Naomi
YAMASHITA Mei
OKAMOTO Maiko
BHOR Sachin Ashok
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | 植物ウイルス病 / 遺伝子組換え植物 / 活性酸素種 / 葉緑体 |
Outline of Final Research Achievements |
The present study aimed to generate plants tolerant to plant virus diseases. To this end, we designed a genetically modified plant system to express timely the gene(s) regulating the reactive oxygen species (ROS) production in chloroplasts, which is known to have roles in plant disease development. We established a cheap method to introduce foreign genes into chloroplast and introduced genes that can regulate ROS production into tobacco chloroplasts. Through the examination of a switching gene that turns on the foreign gene in chloroplasts, we found that both protein and RNA structures are important to enable the switching gene product for its efficient transport into chloroplasts.
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Free Research Field |
植物ウイルス病の発症機構の解析と耐病性分子育種
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