Control method of Bemisia tabaci using siRNA specific to its PolA1 gene

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14669
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Chiba University
• Principal Investigator: Nakamura Ikuo 千葉大学, 大学院園芸学研究科, 教授 (50207867)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: siRNA / pest-control / PolA1 / tobacco whitefly / VIGS / HIGS

Outline of Final Research Achievements

Tobacco whitefly is an agricultural pest that greatly damages by sucking sap and spreading virus. Recently a race that is resistant to insecticides has emerged, and thus its control becomes a major problem.
In this study, we aimed to develop new control method of tobacco whitefly using siRNA, and constructed vectors for generating siRNA targeting the species-specific sequence of tobacco whitefly PolA1 gene. In tobacco plants inoculated Agrobacterium containing viral vectors, hatching of tobacco whitefly eggs is significantly inhibited compared with control plants, although more detail analysis will be necessary. N.B. siRNA (small interfering RNA): inhibits expression of gene that contains homologous sequence with siRNA.

Free Research Field

植物分子育種学