2017 Fiscal Year Final Research Report
Investigation and application of highly selective bromination reaction mechanism of biosynthetic enzyme of Tyrian purple, an ancient pigment
| Project/Area Number |
15K14709
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SAKURAI Takeshi 金沢大学, 物質化学系, 教授 (90116038)
HAYASHI Yoshihito 金沢大学, 物質化学系, 教授 (10231531)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | bromoperoxidase / haloperoxidase / Tyrian purple / shellfish |
|---|
| Outline of Final Research Achievements |
Tyrian purple having a molecular structure of 6,6'-dibromoindigo is a rare animal natural dye used since 1,600 BC. In this study, we tried to purify bromoperoxidase (BPO), which catalyzes the bromination reaction of purple pigment precursor from the hypobranchial gland of the shellfish, Rapana venosa. Since BPO exists only in trace amounts in shellfish, it could not be purified homogeneously, but revealed that a soluble activating protein is necessary for the activity of BPO present in the membrane fraction. BPO composed of two components is a novel enzyme, which has never been seen before.
|
| Free Research Field |
応用生物化学
|
