2017 Fiscal Year Final Research Report
Identification of novel demyristoylation enzyme controlling lipid modification cycle and its structural function analysis
| Project/Area Number |
15K14712
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto Gakuen University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ミリストイル化 / ミリスチル化 / 脂質修飾 / 細胞内シグナル伝達 / 脱ミリストイル化酵素 |
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| Outline of Final Research Achievements |
Protein myristoylation is an irreversible lipid modification and demyristoylation enzymes have not yet been identified. Since many myristoylated proteins are important for disease pathogenesis, it is a major finding to clarify the presence of demyristoylation enzymes involved in the regulation of myristoylated proteins.
In this study, we aimed to purify and identify the demyristoylation enzyme and to clarify its enzymatic caharacteristics. As a result, we succeeded in finding demyristoylation activity from rat brain soluble fraction. In this research process, we also developed an assay system to identify demytostroylation activity.
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| Free Research Field |
蛋白質科学、細胞生物学、分子生物学、構造生物学
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