Population genetic analysis for identifying hybrid origin of dwarf bamboo species using next-generation sequencing

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14745
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Tohoku University
• Principal Investigator: Suyama Yoshihisa 東北大学, 農学研究科, 准教授 (60282315)
• Co-Investigator(Renkei-kenkyūsha): MAKITA AKIFUMI 秋田県立大学, 生物資源科学部, 教授 (60315596)
• Research Collaborator: YONEKURA KOJI 東北大学, 植物園, 助教 ; KANNO TAKAMASA 東北大学, 大学院農学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 雑種識別 / MIG-seq法 / 集団遺伝学 / NGS / ササ / ホンシャクナゲ

Outline of Final Research Achievements

Studies of the genetic origin of hybrids are required to clarify systematic relationships in many species groups. However, it is generally difficult to develop common DNA markers among different species for the genetic analysis. Here, we conducted a new approach, multiplexed ISSR genotyping by sequencing (MIG-seq) method, for identifying the parental species of two putative hybrid species (groups) of dwarf bamboo and Rhododendron. Our population genetic analysis strongly suggested hybrid origin of the species. This approach can be applied to investigating a wide range of hybrid species, and will be able to contribute to systematic studies of many species.

Free Research Field

森林分子生態学