2016 Fiscal Year Final Research Report
Development of artificial enzymes bearing lignin-binding peptide sequence and their delivery system
| Project/Area Number |
15K14773
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | リグニン / ペプチド / ラッカーゼ / バイオリファイナリー / バイオマス / リグニン分解酵素 |
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| Outline of Final Research Achievements |
The binding behavior of a 12-mer peptide and its tandem dimer to lignin was analyzed. The tandem dimerization increased affinity to lignin by around 10 times. The tandem dimer significantly changed its conformation upon addition of the lignin to have a 10-times higher affinity than the 12-mer peptide. Laccase bearing the tandem dimer peptide was expressed, and reacted to woody biomass pretreated by microwave hot water treatment. It was found that the incorporation of lignin-binding peptide increased the reactivity to lignin and gave higher amount of low molecular mass fragments.
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| Free Research Field |
バイオマス変換
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