Metagenetic analysis on symbiotic bacterial community with Daphnia colonizing near a vent on the lake floor of Lake Biwa

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14783
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: Ban Syuhei 滋賀県立大学, 環境科学部, 教授 (50238234)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 環境微生物 / ミジンコ / 共生微生物 / 琵琶湖 / ベント孔

Outline of Final Research Achievements

Daphnia spp. were collected from 0-30 m and 60-90 m at a station (90 m deep) in North basin of Lake Biwa. Archaeal and bacterial communities associated with whole bodies, guts and feces were separately analyzed with a molecular technique. Whole DNAs were extracted from each of the all samples with a Soil DNA Isolation Kit. Then, library of each sample was made with two-step PCR targeted to V4 region of 16S rDNA in archaea and bacteria. More than 150 OTUs in genus level were detected from all samples, suggesting extremely high diversity of bacterial communities related with Daphnia bodies, guts and feces. No archaea were found, and therefore no methane-oxidizing bacteria related food-web was shown in this study. According to cluster analysis in order level, bacterial compositions in whole bodies, guts and feces were separated to different clusters, showing specific bacterial communities in each of the three compartments tested.

Free Research Field

水圏生態学