Efficient search for cell cycle inhibitors from marine organisms by usinf Fucci cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14799
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: The University of Tokyo
• Principal Investigator: Matsunaga Shigeki 東京大学, 大学院農学生命科学研究科(農学部), 教授 (60183951)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 海洋生物 / 細胞周期

Outline of Final Research Achievements

By using HeLa/Fucci2 cells, it is possible to observe the progression of cell cycle in live cells under microscope, enabling the differentiation of non-specific toxic compounds from specific cell-cycle inhibitors. We applied this technology to search for compounds that affect the cell-cycle of the HeLa/Fucci2 cells. From the brittle star collected at Ishigaki Island we isolated and characterized curacin E and from the marine sponge collected at Kurose, north to Hachijo Island, dragmacidins G and H, pyrazines substituted by two indoles and a guanidinoethylthio group.

Free Research Field

海洋天然物化学