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2016 Fiscal Year Final Research Report

The exploration of functional domain of IFN-tau with synthetic peptides for the possible substitution of recombinant protein

Research Project

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Project/Area Number 15K14837
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Animal production science
Research InstitutionHokkaido University

Principal Investigator

Masashi Takahashi  北海道大学, 農学研究院, 教授 (10343964)

Project Period (FY) 2015-04-01 – 2017-03-31
Keywordsインターフェロンτ / 子宮内膜細胞
Outline of Final Research Achievements

The purpose of the present research is to search the activities of synthetic peptides constituting IFN-τ for a possible substitution for recombinant IFN-τ. Eleven peptides (long chain: 27-28 aa, short chain: 7-17 aa) have been chemically synthesized from amino acid sequence and structure of IFN-τ. Each or mixed peptides or recombinant IFN-τ were added to cultured bovine endometrium stromal cells to detect the expression of MX1, 2 and ISG15 that belong to interferon-stimulated genes (ISGs). Next, each or mixed peptides were added to the cells with recombinant IFN-τ and expression of ISGs was evaluated.IFN-τ significantly stimulated ISGs expression, whereas all single or mixed peptides did not. Addition of single or mixed peptides did not show the inhibitory effect of IFN-τ.These result suggest that synthetic peptides would not have agonist and antagonist ability or binding to IFN receptor by the possible structural change.

Free Research Field

家畜繁殖

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Published: 2018-03-22  

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