Functional analysis of Exosome in Canine Tumor

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14872
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: Miura Naoki 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (80508036)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: マイクロRNA / エキソソーム / イヌ / 腫瘍 / 次世代シーケンス

Outline of Final Research Achievements

In the present study, we seek the new molecular bio-marker for diagnosis and treatment of dog tumor. We focus specially exosome molecule which has been identified as small follicle released from tumor. We successfully isolated exosome from dog blood by ultra centrifuge. Isolated dog exosome express surface antigen as similar as human exosome. We also investigated the expression of micro RNA in dog tumor. Micro RNA has been intensively focused in human cancer biology. We can successfully identified the unique microRNAs in several dog tumor. Finally, we can measure the microRNA expression in exosome by quantitative real-time PCR.

Free Research Field

獣医画像診断