Understanding the mechanism of loss of mouse spermatogenic stem cell clones following transplantation.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14890
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Yoshiaki Nakamura 基礎生物学研究所, 生殖細胞研究部門, 特別研究員 (30613723)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 精子幹細胞 / マウス / 移植 / 大量死 / 分化

Outline of Final Research Achievements

Spermatogenic stem cells (SSCs) can settle then reconstitute seminiferous epithelium following transplantation into infertile recipients. This study aimed to understand the cause of massive death of mouse SSC clones after settlement. Live-imaging study revealed that SSCs undergo cell death after settlement. Fate analysis suggested that major part of SSC clones undergo differentiation after settlement. Subsequent clonal fate analysis of SSCs in the testes of vitamin A-deficient recipient mice, that is expected to inhibit SSC differentiation, suggested that SSC clones undergo cell death after clonal differentiation.

Free Research Field

発生工学