Study of allosteric regulation of solute transporter by a second-messenger and its structural analysis.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14911
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: ABE Keietsu 東北大学, 農学研究科, 教授 (50312624)
• Co-Investigator(Renkei-kenkyūsha): NANATANI Kei 東北大学, 大学院工学研究科, 助教 (00547333) ; OGASAWARA Satoshi 東北大学, 医学系研究科, 助教 (30546685)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 輸送体 / 物質生産 / 構造解析

Outline of Final Research Achievements

Aspartate:alanine antiporter AspT and its orthologues are industrially important solute exporters in bacteria. We established expression and purification systems for AspT with high yield and purity. We screened thermo-stable conditions for AspT in the presence of several ligands including substrates and their analogs. We performed crystallization of AspT and obtained some crystals at several optimized conditions. We collected X-ray diffraction data of AspT with the resolution of 4-6 Å, and are trying to improve molecular packing of crystals of AspT for better resolution.

Free Research Field

応用微生物学