2017 Fiscal Year Final Research Report
Development of the method for generation of transplastomic plants harboring artificially designed plastid genomes
| Project/Area Number |
15K14912
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 葉緑体 / 合成生物学 / 人工ゲノム / 遺伝子発現制御 / 人工リボスイッチ / 人工ヌクレアーゼ |
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| Outline of Final Research Achievements |
Towards comprehensive design of chloroplast genomes in higher plants, in this work, we attempted to develop the method for allowing complete replacement of the wild-type plastid genomes with artificially constructed ones. Firstly, we established an engineered riboswitch-based inducible gene expression system for strict regulation of plastid genes. Then, we examined whether a riboswitch-controlled artificial endonuclease, exclusively recognizing specific DNA sequences derived from the wild-type plastid genomes, could help to achieve homoplasmy of transplastomic lines.
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| Free Research Field |
植物分子生物学
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