2016 Fiscal Year Final Research Report
Construction of a novel fluorescent measurement system to evaluate pH values in fluorescent imaging analysis
| Project/Area Number |
15K14914
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MISAKA Takumi 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (40373196)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 培養細胞 / pH / 蛍光イメージング |
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| Outline of Final Research Achievements |
We planned to construct a novel evaluation system to detect a decrease in pH value of extracellular solution by fluorescent imaging analysis. Extracellular pH decease could be clearly detected by the change of fluorescent intensities of EGFP by using cultured cells that express both a novel proton channel and EGFP. It was also shown that sensitivities of sensor cell lines against pH decrease depend on the expression level of a novel proton channel.
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| Free Research Field |
食品科学
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