Building up the bioluminescence molecule on the nucleic acid sequence and its application to gene detection technology

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14933
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KOJIMA Naoshi 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30356985)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 遺伝子検出 / 生物発光 / ルシフェリン / 核酸プローブ / 核酸化学

Outline of Final Research Achievements

In this research, I focused on the to development of a novel gene detection system by utilizing "bioluminescence", which was unexplored in the field of gene detection technology. To achieve this goal, I carried out the following two studies. In the first study, I developed a system to convert sequence information into bioluminescence signal. I designed and chemically synthesized novel nucleic acid probe that releases a bioluminescent substrate upon binding to the target sequence. In the second study, I have developed the method for introducing guanidine nucleic acid, which possesses positive charges instead of negative charges, into oligonucleotide with the aim to strengthen the function of nucleic acid probe.

Free Research Field

核酸化学、生物有機化学