2016 Fiscal Year Final Research Report
Development of chitosan-coated DNA microgels for peroral delivery of nucleic acid drugs
| Project/Area Number |
15K14939
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 核酸 / DNA / ハイドロゲル / 経口投与 / キトサン |
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| Outline of Final Research Achievements |
To develop nucleic acid-based therapeutic systems for inflammatory bowel diseases, we tried to develop chitosan-coated DNA microgels, which prevent the intestinal degradation and delivery to the lower intestine, the main target of the diseases, of nucleic acids, based on the “self-gelatinizable nucleic acid” technology. The applicability of DNA hydrogel to mucosa was first examined using DNA hydrogel harboring CpG1668, an immunostimulatory DNA. Intravaginal administration of CpG1668/DNA hydrogel resulted in high cytokine production and efficient inhibition of the growth of simplex herpes virus type 2. Then, chitosan-coated DNA microgels were developed. Chitosan-coating significantly increased the stability of DNA hydrogel in acidic solutions or DNase-containing solutions. These results indicate that the chitosan-coated DNA microgels developed in this study will lead to the development of nucleic acid-based therapeutic systems for inflammatory bowel diseases.
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| Free Research Field |
生物薬剤学
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