2016 Fiscal Year Final Research Report
Cell surface modification using non-natural amino acid and its application to drug delivery system
| Project/Area Number |
15K14940
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 細胞膜修飾 / 非天然アミノ酸 / VHH / 細胞接着 / 細胞製剤 |
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| Outline of Final Research Achievements |
The purpose of this study is to enhance the cell-cell adhesion by introducing the protein ligand via PEG-lipid to cells. Variable domain of heavy chain of heavy chain antibody (VHH) against mCherry was used as a model protein ligand. Azide group was introduced into VHH at the selected position as the connection of PEG-lipid through azide group does not disturb antigen binding. VHHs were modified on the cell surface by adding Az-VHH after cells were coated with DBCO-PEG-DSPE. VHH modified on the cell surface could specifically bind with antigen. Adhesion to antigen-coated plate was enhanced by VHH-modification to cells.
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| Free Research Field |
ドラッグデリバリー
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