Development of de novo phasing method with XFEL toward a structure determination of a drug target protein

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K14941
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: Nakatsu Toru 京都大学, 薬学研究科(研究院), 准教授 (50293949)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: X線自由電子レーザー / X線結晶構造解析 / タンパク質 / 重原子同型置換法 / 異常分散効果

Outline of Final Research Achievements

We need to understand the drug target protein in order to study the drug discovery research. It is important to determine the protein structure with X-ray crystallography. However it is difficult to determine the protein structure because the size of the crystal is only 1~10 μm. The structural information is obtained from small crystals illuminated by an X-ray free electron laser.
Then we developed de novo protein structure determination method using μm-sized protein crystals at SACLA which is Japanese X-ray free electron laser fcillity.

Free Research Field

タンパク質結晶学