Development of pH-sensitive flagellar driven type-liposomes penetrable into tumor tissue

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14945
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: The University of Tokushima
• Principal Investigator: KOGURE Kentaro 徳島大学, 大学院医歯薬学研究部(薬学系), 教授 (70262540)
• Co-Investigator(Renkei-kenkyūsha): GOTO Naomasa 京都薬科大学, 薬学部, 学長 (30121560)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ドラッグデリバリーシステム

Outline of Final Research Achievements

In this study, we tried to develop DDS carriers, which can sense pH change and move autonomously in tumor environment. In the first year, we found that detailed consideration of conditions was required for preparation of bacterial ghost, and succeeded in isolation of flagellar. In the second year, we succeeded in the preparation of bacterial ghost. In addition, we confirmed the existence of flagellar filament component protein, FliC, in the ghost. Based on these results, we concluded that we succeeded in the preparation method of bacterial ghost. Furthermore, we developed the nanoparticles, of which surface was modified with tumor penetrable functional peptide AT1002 and polyethyleneglycol, and succeeded in observation of penetration of the nanoparticles into cancer cell spheroids.

Free Research Field

薬物送達学、生物物理化学